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. 2020 Sep 11;11:4557. doi: 10.1038/s41467-020-18425-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a In apo-CA II, the metal binding site is vacant. b, c Zn- and Co-CA II show tetrahedral, d Ni-CA II octahedral, and e Cu-CA II trigonal bipyramidal coordination geometry. The electron density (2F_o–F_c, blue) is contoured at 2.2σ. All structures were obtained at pH 7.8 except for (c) which is obtained at pH 11.0. The intermediate water (W_I) in (d) is colored in steel blue for clarity.