Skip to main content
. 2020 Aug 27;15(3):612–628. doi: 10.1016/j.stemcr.2020.07.022

Figure 6.

Figure 6

Activation of YAP/TAZ Abolished the Effects of ROCK and Myosin II Inhibitors on Cardiac Reprogramming

(A and B) FACS analysis of cTnT expression after 1 week. GHMT-transduced cells cultured on 126 kPa hydrogels and treated with ROCK inhibitor were transduced with YAP-5SA and TAZ-4SA. Activation of YAP/TAZ suppressed ROCK inhibitor-mediated cardiac reprogramming. (B) Quantitative data; n = 3 independent triplicate experiments.

(C and D) FACS analysis of cTnT expression after 1 week. GHMT-transduced cells cultured on 126 kPa hydrogels and treated with myosin II inhibitor were transduced with YAP-5SA and TAZ-4SA. Activation of YAP/TAZ suppressed myosin II inhibitor-mediated cardiac reprogramming. (D) Quantitative data; n = 3 independent triplicate experiments.

(E, F, H, and I) Immunocytochemistry of αMHC-GFP, cTnT, and DAPI after 4 weeks. GHMT-fibroblasts cultured on 126 kPa hydrogels and treated with ROCK inhibitor for 4 weeks (E and F) and myosin II inhibitor for first 2 weeks (H and I) were transduced with YAP-5SA and TAZ-4SA. (F and I) Quantitative data; n = 3 independent triplicate experiments.

(G and J) Quantitative data of the number of spontaneously beating cells after 4 weeks (n = 4 independent triplicate experiments). Activation of YAP/TAZ suppressed ROCK (G) and myosin II inhibitor (J)-mediated cardiac reprogramming.

All experiments were performed on 126 kPa hydrogels. All data are presented as the means ± SD. ∗p < 0.05 versus the relevant control. Scale bars represent 100 μm.