Figure 7.

Soft Matrix Promoted SeV-Mediated Cardiac Reprogramming via YAP/TAZ Suppression

(A) Quantification of the spontaneously beating iCMs after 4 weeks of culture (n = 4 independent triplicate experiments). Fibroblasts were seeded on PS or 8 kPa hydrogels and transduced with pMX-GMT or SeV-GMT. Soft substrate enhanced SeV-mediated cardiac reprogramming.

(B) Immunocytochemistry of α-actinin and DAPI after 4 weeks. Cell were treated as shown in (A).

(C) Quantification of data presented in (B); n = 3 independent triplicate experiments.

(D) Western blot analysis for GMT, YAP/TAZ, and pYAP/pTAZ protein expression in pMX-GMT or SeV-GMT-transduced fibroblasts cultured on 8 kPa hydrogels; n = 3 independent biological replicates.

(E) Quantification of the number of spontaneously beating cells after 4 weeks; n = 4 independent triplicate experiments. Activation of YAP/TAZ suppressed SeV-mediated cardiac reprogramming.

(F) Immunocytochemistry of α-actinin and DAPI after 4 weeks.

(G) Quantification of data presented in (F); n = 3 independent triplicate experiments.

All data are presented as the mean ± SD. ^∗p < 0.05; ^∗∗p < 0.01 versus the corresponding control. Scale bars represent 100 μm.