Figure 1.

Schematic of Assay Developed for Neuroblast Migration

(A) The RMS (dark pink) is dissected from slices and single-cell dissociated.

(B and C) (B) Cells are plated in round bottom plates and (C) cells sink to bottom and form spheroids.

(D and E) (D) Matrigel is added to the spheroids and (E) neuroblasts emigrate from them in 3D. The image shows the spheroid (encircled in yellow) at 24 h with the emigrated cells manually outlined in purple to determine emigration surface area. Scale bar, 100 μm.

(F) Migration is imaged and measured at various time points.