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Workflow of Automated Spheroid Image Analysis
(A) Examples of typical bright-field, Hoechst (live stain) and propidium iodide (dead stain) images used in our analysis.
(B) The automated analysis sequence describes the imaging sequence and the processing carried out by the analysis software.
(C) Neuroblast numbers within increasing radius zones from the spheroid were measured by the software to determine migration extent.
(D) Errors affecting the counts were checked automatically.
