Fig. 4.

Chelerythrine inhibited the biofilm formation of mono- and dual-species of S. aureus ATCC 25,923 and S. lugdunensis ATCC 700,328 on slides. The biofilms of dual-species formed on glass slides were inhibited by chelerythrine of different concentrations (0, 1/8 MIC, 1/4 MIC and 1/2 MIC). a, d, g Samples stained with crystal violet were observed by optical microscopy at the magnification of 40 × (scale bar: 2.5 μm). b, e, h CLSM images. (scale bar: 10 μm) (c, f, i) Photographs of environmental scanning electron microscope (ESEM, magnification of 10,000 × , scale bar: 10 μm)