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. 2020 Sep 11;11(9):744. doi: 10.1038/s41419-020-02941-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a HeLa cells expressing MLKL shRNA, or a non-silencing control were analyzed by western blotting, and these cells were treated with varying doses of TRAIL for 12 h and cell viability was analyzed by MTT assay (upper panel) or phase-contrast microscopy (bottom panel). The results are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 100 μm. b HeLa cells stably expressing MLKL shRNA or non-silencing control were treated with TRAIL (0.6 ng/ml) in a time-dependent manner (upper panel), and these cells treated for 5 h in dose-dependent manner (bottom panel). The cells were harvested, and total lysates were analyzed by western blotting. c A549, HCC4006, H2009, and MDA-MB231 cells expressing MLKL shRNA, or non-silencing control were analyzed by western blotting (upper panel), and these cells were treated with varying doses of TRAIL for 24 h and cell viability was analyzed by MTT assay (bottom panel). The results are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.