Fig. 3. Combined blockade of GARP:TGF-β1 and PD-1 shows anti-tumor efficacy against established CT26 tumors.

BALB/c mice were injected s.c. with live CT26 cells on day 0. Tumor diameters were measured two to three times a week. On day 6, mice were randomized in various experimental groups and received the first of 3–4 mAb injections. Mice were euthanized when the tumor surface was ≥200 mm². a Meta-analysis of four pooled independent experiments in which mice received anti-PD-1 WT alone, or in combination with anti-GARP:TGF-β1 WT (n = 39). b Meta-analysis of three pooled independent experiments in which mice received anti-PD-1 FcS alone, or in combination with anti-GARP:TGF-β1 FcD (n = 29–30). a, b Kaplan Meier plots on top represent the proportions of mice alive during the experiment, with P values calculated using a one-tailed Wilcoxon test. Graphs on bottom represent evolution of tumor volumes in various groups (each line represents one mouse). Ratios indicate the proportions of CR and PR, as defined in Fig. 2. Rectangles on the right represent proportions (%) of CR (filled rectangles), and partial + non-responders (PR + NR, empty rectangles).