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. 2020 Sep 11;5:85. doi: 10.1038/s41541-020-00231-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunoreactivity of the IVTT-expressed denatured E. ca. hypothetical proteins compared with TRP19 by ELISA. The IVTT proteins reacted with convalescent sera from 10 CME dogs. b Immunoreactivity of overlapping synthetic peptides spanning 3 E. ca. immunoreactive proteins, as determined by ELISA with a CME dog serum. Positive control, TRP19. ELISA OD values represent the mean optical density reading from three wells (±standard deviations) after background subtraction. A sample OD of ≥0.1 was considered positive and ≥0.5 a strong positive after subtracting negative control (a: IVTT reaction with empty plasmid template and b: a negative peptide) reading.