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. 2020 Sep 11;11:4576. doi: 10.1038/s41467-020-18413-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Domain structure of mouse PDZD8(WT) and PDZD8(ΔTM). b Schematic representation of the liposome-FRET assay. c Phospholipid (PA, PS, PE, and PC) extraction activities of GST-PDZD8(ΔTM). d Ceramide (left) or cholesterol (right) extraction activity of GST-PDZD8(ΔTM). e Initial velocity of lipid extraction by GST-PDZD8(ΔTM) measured in c and d). f Phospholipid extraction activities of GST-PDZD8(ΔTM) with PA, PS, PE, and PC in the absence (−) or presence (+) of acceptor liposomes. g Concentration dependence for lipid extraction activity of GST-PDZD8(ΔTM) with PS in the absence (left) or presence (right) of acceptor liposomes. h Initial velocity of lipid extraction by GST-PDZD8(ΔTM) measured in g. i Detection of lipid (PS) extracted by GST-PDZD8(ΔTM) in the supernatent (Sup) separated from liposomes in the pellet after ultracentrifugation of the reaction mixture at 300 s.