Fig. 4. A-485 suppresses hepatic lipogenesis.

a Oil red O staining of liver sections from normal chow diet (NCD)-fed mice treated with vehicle and A-485 for 7 days (bar = 100 μm). b, c Hepatic triglyceride of NCD-fed and high-fat diet (HFD)-fed mice. d, e Hepatic total cholesterol contents of NCD-fed and HFD-fed mice. f Fatty acid synthase (FAS) and carbohydrate-responsive element-binding protein (ChREBP) protein expressions in the liver from NCD-fed mice. g mRNA expressions of fatty acid oxidation-related genes in the liver from NCD-mice. h–j Primary mouse hepatocytes were incubated with 3 μM A-485 for 18 h in the presence of 0 or 100 nM insulin and 5 or 25 mM glucose. Lipogenesis- and fatty acid oxidation-related gene expressions were detected. Data are expressed as means ± SEM (n = 5–9). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. corresponding control group; ^#P < 0.05.