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. 2020 Sep 11;3:507. doi: 10.1038/s42003-020-01224-5

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© The Author(s) 2020, corrected publication 2025

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Extracted ion chromatograms (EICs) for m/z 136 corresponding to the [M+H]⁺ adduct of authentic phenylacetaldoxime (upper panel), metabolite extracts from N. benthamiana leaves expressing PaqFOS1 (middle panel) and empty vector control (lower panel). b m/z 318 EICs corresponding to the [M+Na]⁺ adduct of an authentic prunasin standard (upper panel), metabolite extracts from N. benthamiana transiently expressing PaqFOS1 in combination with PdCYP71AN24 and PdUGT85A19^4,14 (middle panel) and the control expressing PdCYP71AN24 and PdUGT85A19 (lower panel). c Base peak chromatograms (BPCs) of the metabolite extracts from N. benthamiana leaves expressing PaqFOS1 using expression of p19 as an empty vector control show the formation of additional products: m/z 320 at 6.7 min corresponds to the [M+Na]⁺ adduct of glucosylated phenylacetaldoxime; m/z 406 at 7.6 and 7.9 min correspond to the [M+Na]⁺ adduct of a glycosylated, phenylacetaldoxime-malonic acid conjugate; and m/z 393.11 at 8.1 min correspond to the [M+Na]⁺ adduct of phenylethanol glucoside malonate ester^2,10. For MS/MS of additional products, see Supplementary Fig. 3.