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. 2020 Sep 11;11:4559. doi: 10.1038/s41467-020-18432-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Quantification of the expression of single molecular markers in CA1 ebGABA. SOM: n = 4 mice; PV: n = 5 mice; NPY: n = 5 mice; M2R: n = 3 mice; nNOS weak/strong: n = 5 mice. Bar plots represent means. Error bars represent SDs. b Example ebGABA in stratum oriens expressing SOM and strong levels of nNOS (arrow). Note that neurons expressing weak levels of nNOS (arrowheads) are SOM immunonegative. Experiment repeated independently in four mice with similar results. c EbGABA in stratum radiatum expressing only M2R but not nNOS (arrow). Experiment repeated independently in three mice, obtaining similar results. d Neurolucida reconstructions of representative neurobiotin-filled ebGABA in the adult CA1. Axon is depicted in green. Soma and dendrites are colored in magenta. Inset: zoomed out representation to illustrate the rostro-caudal and dorso-ventral position. e Representative responses to +20, +520, and +820 pA current injections in ctrlGABA and ebGABA. Note the lower firing rate of the ebGABA in response to stronger current injections. f Top, f/I plot showing reduced excitability of ebGABA with strong current injections. Effect of current injection F(14,864) = 31.59, P < 0.0001; effect of birth date F(7,864) = 16.40, P < 0.0001, interaction F(98, 864) = 1.881 P < 0.0001, two-way ANOVA with Bonferroni post hoc test. Bottom, f/I plot limited to the first 50 ms of the current injection. Effect of current injection F(14,461) = 77.8, P < 0.0001; effect of birth date F(1,461) = 3.211, P = 0.0738, interaction F(1,461) = 1.676, P = 0.0573, two-way ANOVA with Bonferroni multiple comparisons test. CtrlGABAs: n = 18; ebGABAs: n = 16. Data are represented as means ± standard errors of the means. g Representative sag response of a ctrlGABA (black trace, −500 pA current injection) and an ebGABA (green trace, −250 pA current injection). Each trace is an average of three sweeps. h EbGABAs (n = 16 cells) display a significantly higher sag ratio (i.e., a lower sag amplitude) than ctrlGABAs (n = 18 cells, P = 0.028, two-sided Mann–Whitney U test). Boxplots represent medians (center), interquartile ranges (bounds), minima and maxima (whiskers). *P < 0.05; **P < 0.01; ***P < 0.001. so stratum oriens, sp stratum pyramidale, sr stratum radiatum, slm stratum lacunosum-moleculare. Source data are provided as a Source Data file.