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. 2020 Sep 11;11:4559. doi: 10.1038/s41467-020-18432-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Example voltage clamp traces showing lower sIPSC frequency in a ebGABA cell than in a ctrlGABA cell. b sIPSC frequency but not amplitude is significantly lower in ebGABAs (n = 17 cells) than ctrlGABAs (n = 18 cells; P = 0.043, two-sided Mann–Whitney U test). c Representative IPSCs evoked by local electrical stimulation. IPSCs evoked in the ctrlGABA cell display higher amplitude than the ones evoked in the ebGABA cell. Stimulation artifacts were trimmed for clarity. d The maximum amplitude of the IPSC evoked by local electrical stimulation is significantly lower in ebGABAs than ctrlGABAs (P = 0.0085, Mann–Whitney U test, ctrlGABAs n = 10, ebGABAs n = 8). e IPSC paired pulse ratio is not statistically different between ctrlGABAs and ebGABAs (P = 0.792, two-sided Mann–Whitney U test, ctrlGABA n = 10, ebGABA n = 8). f Representative IPSCs evoked by optical stimulation of medial septal terminals. IPSCs evoked in ctrlGABA and ebGABA cells display similar amplitudes. g, h No significant difference between ebGABAs (n = 12 cells) and ctrlGABAs (n = 13 cells) in maximum amplitude (P = 0.611, two-sided Mann–Whitney U test) and paired pulse ratio (P = 0.796, two-sided Mann–Whitney U test) of the IPSCs evoked by optical stimulation of septal terminals. i Representative maximum intensity projections of confocal z-stacks showing innervation of a ctrlGABA and an ebGABA by PV+ boutons. Appositions between PV+ boutons and GFP+ somata or dendrites are highlighted with arrows; lack of apposition is marked with dashed arrowheads. Insets: single optical sections (thickness 0.41 µm) showing magnifications of the appositions. Left, z-stack depth 20.5 µm. Right, z-stack depth 15.17 µm. Experiment repeated independently for 47 ctrlGABAs and 30 ebGABAs with similar results. j EbGABAs display a significantly lower number of PV+ appositions than ctrlGABAs (top, P < 0.0001, two-sided Mann–Whitney U test, n = 47 ctrlGABAs, n = 30 ebGABAs); area of the PV staining is not significantly different between the two populations of cells (bottom, P = 0.236, two-sided Mann–Whitney U test, n = 47 ctrlGABAs, n = 30 ebGABAs). Boxplots in panels b, d, e, and g, h represent medians (center), interquartile ranges (bounds), minima and maxima (whiskers). In panel j, data are represented as scatter plots with medians and interquartile ranges. *P < 0.05. **P < 0.01. ****P < 0.0001. Source data are provided as a Source Data file.