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. 2020 Sep 6;6(9):e04851. doi: 10.1016/j.heliyon.2020.e04851

Figure 4.

Figure 4

The morphological effects of CM derived from SH-SY5Y cells treated with polyQ on PC12 cell after differentiation. (A) Time schedule of differentiation of PC12 cells and treatment with CM from SH-SY5Y cells treated with vehicle, LPS, 15Q or 69Q as Figure 3. (B, C, D) Quantification of the area of cell body, total length of neurites and/or number of branch point of neurites of PC12 cells after differentiation in the presence of CM from SH-SY5Y cells treated with vehicle (n = 95 cells) (B, C, D), LPS (n = 89 cells) (B, C), 15Q (n = 89 cells) (B, D) or 69Q (n = 75 cells) (B, D) (each from 3 experiments). Representative images of PC12 cells are shown in (B). Error bars represent SE. Scale bars, 20 μm.