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. 2020 Sep 6;6(9):e04851. doi: 10.1016/j.heliyon.2020.e04851

Figure 5.

Figure 5

The morphological effect of CM derived from BV2 cells treated with polyQ on PC12 cell during differentiation. (A) Time schedule of differentiation of PC12 cells and treatment with CM from BV2 cells treated with vehicle, LPS, 15Q or 69Q during differentiation. (B, C, D) Quantification of the area of cell body, total length of neurites and/or number of branch point of the neurites of PC12 cells during differentiation in the presence of CM from BV2 cells treated with vehicle (n = 99 cells) (B, C, D), LPS (n = 70 cells) (B, C), 15Q (n = 99 cells) (B, D) or 69Q (n = 99 cells) (B, D) (each from 3 experiments). Representative images of PC12 cells are shown in (B). Error bars represent SE. ANOVA, ∗∗p < 0.01. Scale bar, 20 μm.