Figure 6.
Combination Bicalutamide and J54 Suppresses Growth of LNCaP Xenografts
(A) Time course of tumor growth of LNCaP cells xenografts in 4 treatment groups. Treatment started 19 days after implantation when the tumors measure ~200 mm3. Two independent experiments with 5 mice per group were carried out. J54 and bicalutamide were dissolved in DMSO and diluted in corn oil 1:10 and administered intraperitoneally biweekly. Sectioning and processing of the tissues were carried out in the FWCC Histology Service, using automated processes and equipment to provide uniform and standardized results. Indirect labeling was with ABC Elite: RTU Vectastain Elite Reagent, Vector #PK-7100; DAB: ImmPact DAB, Vector #SK-4105. Light counterstaining was done with hematoxylin.
(B) Tumor weights were determined for all groups at end of the treatment course.
(C) Examples of tumor size at the end of the experiment.
(D) Representative sections from tumors resected from mice in the 4 treatment groups analyzed by immunohistochemistry for pNek1 (n = 3 mice; scale bar, 50 μm). Note the weak pNek1 stain in the tumor from mice treated with J54, in contrast to the increase seen with BIC.
Two-way ANOVA tests were done to compare the group for statistical significance; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.