Fig. 3.

Optimization of A. tumefaciens concentration (A) Typical phenotype of N.benthamiana leaves expressing hEGF infiltrated with different Agrobacterium concentration (0.2, 0.4, 0.6, and 0.8 at OD₆₀₀) on 2, 4 and 6 dpi. (B) Western blot of harvested leaves (4 dpi) infiltrated with varying concentrations of A. tumefaciens under reducing condition. The membrane was probed with HRP-conjugated anti-His antibody. kDa: kilodalton; OD₆₀₀:optical density at 600 nm; dpi: day post-infiltration.; the lane number represents the OD₆₀₀ of Agrobacterium cells used for agroinfiltration. Arrowhead represents plant-produced hEGF. Equal amounts of total soluble protein were loaded in each lane.