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. 2020 Sep 5;28:e00524. doi: 10.1016/j.btre.2020.e00524

Fig. 6.

Fig. 6

Effect of ammonium sulfate concentration on protein precipitation and purification: (A) SDS-PAGE analysis of precipitated proteins collected from 30-80 % saturated ammonium sulfate concentration in plant crude extract (B) Western blot of plant-produced hEGF crude extract under reducing condition. The blot was probed with HRP-conjugated anti-His antibody. kDa: kilodalton; T: total soluble protein from plant crude extract; the lane number represents the ammonium sulfate concentration used for precipitating the proteins. Arrowhead indicates RuBisCO (Fig. 6A) and plant-produced hEGF (Fig. 6B).