Fig. 8.

Cell proliferation and cytotoxic effect of plant-produced hEGF in HaCaT cells analyzed by MTT assay (A) After 24 h of treatment with plant-produced EGF (P-EGF) and commercial hEGF (hEGF-control) at the concentration from 5, 10, 50 and 100 ng/mL. Values are shown as mean ± SD of the percentage of HaCaT cell viability. (B) Concentration of hEGF at 50, 100 ng/mL did not affect HaCaT cell proliferation. (C) Visualization of HaCaT co-stained with Hoechst 33,342 and PI dye showed that no apoptotic or necrotic cell death was observed after treatment with hEGF.