Fig. 6.

Brusatol promotes erastin-induced ferroptosis via FOCAD-FAK signaling partially. Both FOCAD-wild type (FOCAD-WT) and FOCAD-knockout (FOCAD-KO) cells were treated with brusatol (50 nM for 16 h in qPCR detection and 50 nM for 24 h in western blot detection). Then the cells were harvested for qPCR (A) and western blot detection (B–C). Besides, the function of brusatol on mitochondrial TCA cycle (D–E) and Complex I activity in mitochondrial ETC (F and Fig. S7) were also evaluated respectively. The ferroptosis model was induced by erastin (5 μM for 24 h) or RSL3 (5 μM for 24 h) treatment, and the cell viability (G), MDA (H), 4-HNE (I), as well as the expression of PTGS2 (J) were measured respectively to evaluate ferroptosis in different groups. Results were expressed as mean ± SD (n = 4), and the P value less than 0.05 was considered statistically significant. In Fig. 6A, *: P < 0.05 compared between two different groups. In Fig. 6C and F, #: P < 0.05 compared with FOCAD-WT + DMSO group. In Fig. 6D–E and 6G-6J, *: P < 0.05 compared with the FOCAD-WT group in the first condition. #: P < 0.05 compared between FOCAD-WT group and FOCAD-KO group in same condition. &: P < 0.05 compared with the FOCAD-KO group in the first condition.