Fig. 2.
Identification of the targeting tLyp-1-lamp2b plasmid. (A) Colony PCR image by agarose gel electrophoresis for identification of targeting tLyp-1-lamp2b plasmids. M, 2000 bp ladder marker; 1–4, samples of tLyp-1-lamp2b plasmids. (B) PCR image by agarose gel electrophoresis for identification of the recombinant plasmid double digested with Bgl II and Sal I. M, 2000 bp ladder marker; 1–4, samples of tLyp-1-lamp2b plasmids. (C) Sequencing for tLyp-1 and part of lamp2b genes. The complete plasmid sequencing and comparison results are shown in supplementary.