Fig 2.

Taxonomic differences between Feno groups with T2 endotypes used as covariables across the 22 EDCs show significant enrichment of specifics taxa at the OTU level. Linear discriminant analysis (LDA) and effect size analysis identified bacterial (A) and fungal (B) taxa differentially enriched according to an LDA score cutoff at 2. The colors (dark and gray) represent each Feno group (level cutoff at 25 ppb), and the length represents the LDA score, which is the degree of significant difference (in log scale) for a given taxon. As the order of the numerator and denominator when calculating the effect size is determined by alphabetical order, it is possible to obtain an interpretation of the scale of the difference between 2 groups by using the absolute values of the effect size even though the LDA score is negative for a given taxon. Thus, any taxa colored according to the Feno variable may be interpreted as taxa being significantly increased in abundance compared with the other Feno group.