Skip to main content
. Author manuscript; available in PMC: 2020 Sep 12.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107785. doi: 10.1016/j.celrep.2020.107785

Figure 1. Protein Synthesis Is Required to Maintain Dendritic Surface GABAARs after iLTP.

Figure 1.

(A) Representative dendritic segments of hippocampal neurons labeled with antibodies to surface GABAAR(γ2) and intracellular gephyrin and VGAT. Neurons were fixed at 20, 45, and 90 min post-treatment: iLTP induction, iLTP induction in the presence of cycloheximide (CHX), or CHX alone. Scale bar, 10 μm.

(B) Quantification of cluster area and density from (A). n = 15 or 16 cells per condition. Colored stars compare statistical significance to the control (ctrl).

(C) Hippocampal lysates analyzed at 45 and 90 min post-iLTP induction. Protein levels were normalized to GAPDH; n = 5.

(D) α1, γ2, and gephyrin upregulation can be blocked by cycloheximide (CHX) at 90 min post-iLTP. Protein levels were normalized to GAPDH; n = 5.

All values represent mean ± SEM. *p < 0.05 and **p < 0.01, one-way ANOVA, Bonferroni post hoc test (C) or two-way ANOVA, Bonferroni post hoc test (B and D).