Table 1.
Histaminergic modulation of intrinsic electrophysiological properties of striatal SPNs across postnatal developmentaa
| aCSF | Histamine | p | |
|---|---|---|---|
| P3–P6 | |||
| Resting membrane potential (mV) | –70.73 ± 0.16 | –74.47 ± 0.08 | 1.00E-06 |
| Input resistance (mΩ) | 992.41 ± 65.94 | 647.68 ± 72.79 | 2.22E-04 |
| Membrane time constant (ms) | 36.32 ± 1.56 | 23.90 ± 2.79 | 0.0029 |
| Rheobase (pA) | 26.92 ± 2.56 | 33.76 ± 5.48 | 0.26 |
| Time to first spike (ms) | 168.75 ± 22.83 | 114.89 ± 35.57 | 0.36 |
| Spike rate 50 pA (Hz) | 16.92 ± 1.67 | 11.56 ± 2.06 | 0.16 |
| Spike rate 40 pA (Hz) | 14.58 ± 1.78 | 11.09 ± 2.21 | 0.82 |
| Spike rate 30 pA (Hz) | 11.8 ± 1.52 | 12.5 ± 1.76 | 0.99 |
| Spike rate 20 pA (Hz) | 10 ± 1.29 | 9.68 ± 1.33 | 0.99 |
| First ISI (ms) | 46.72 ± 4.72 | 51.65 ± 5.32 | 0.97 |
| Second ISI (ms) | 51.46 ± 4.84 | 59.05 ± 4.27 | 0.90 |
| Third ISI (ms) | 53.22 ± 2.95 | 55.03 ± 3.13 | 0.98 |
| Fourth ISI (ms) | 56.65 ± 2.57 | 61.54 ± 4.35 | 0.94 |
| First spike amplitude (mV) | 57.82 ± 3.74 | 65.41 ± 2.57 | 0.23 |
| Second spike amplitude (mV) | 50.09 ± 5.02 | 53.31 ± 5.75 | 0.54 |
| First spike duration (ms) | 3.37 ± 0.20 | 3.17 ± 0.19 | 0.88 |
| Second spike duration (ms) | 5.74 ± 0.74 | 4.37 ± 0.21 | 0.06 |
| P9–P12 | |||
| Resting membrane potential (mV) | –73.31 ± 0.07 | –67.35 ± 0.09 | 1.00E-04 |
| Input resistance (mΩ) | 459.84 ± 50.42 | 368.44 ± 28.69 | 0.06 |
| Membrane time constant (ms) | 18.13 ± 1.45 | 16.15 ± 1.17 | 0.22 |
| Rheobase (pA) | 52.27 ± 3.71 | 54.50 ± 4.89 | 0.77 |
| Time to first spike (ms) | 199.82 ± 12.75 | 170.86 ± 23.18 | 0.26 |
| Spike rate 100 pA (Hz) | 22.97 ± 1.66 | 16.97 ± 1.55 | 0.005 |
| Spike rate 80 pA (Hz) | 18.88 ± 1.90 | 13.68 ± 1.42 | 0.02 |
| Spike rate 60 pA (Hz) | 15.33 ± 1.67 | 9.37 ± 1.22 | 0.01 |
| Spike rate 40 pA (Hz) | 8.5 ± 0.91 | 5.35 ± 0.56 | 0.99 |
| First ISI (ms) | 56.8 ± 8.25 | 60.43 ± 9.82 | 0.77 |
| Second ISI (ms) | 52.62 ± 6.61 | 65.66 ± 8.41 | 0.72 |
| Third ISI (ms) | 48.38 ± 5.56 | 61.33 ± 4.08 | 0.42 |
| Fourth ISI (ms) | 41.96 ± 2.73 | 59.29 ± 4.73 | 0.03 |
| First spike amplitude (mV) | 68.53 ± 3.15 | 65.19 ± 2.52 | 0.64 |
| Second spike amplitude (mV) | 63.77 ± 3.21 | 55.75 ± 3.17 | 0.14 |
| First spike duration (ms) | 2.5 ± 0.19 | 2.3 ± 0.06 | 0.49 |
| Second spike duration (ms) | 3.35 ± 0.28 | 3.04 ± 0.11 | 0.33 |
| P21–P35 | |||
| Resting membrane potential (mV) | –70.99 ± 0.21 | –65.96 ± 0.15 | 1.00E-05 |
| Input resistance (mΩ) | 113.44 ± 10.62 | 109.38 ± 10.15 | 0.64 |
| Membrane time constant (ms) | 2.67 ± 0.16 | 3.49 ± 0.37 | 0.008 |
| Rheobase (pA) | 128.71 ± 17.21 | 112.30 ± 19.09 | 0.30 |
| Time to first spike (ms) | 109.70 ± 21.12 | 76.48 ± 15.24 | 0.19 |
| Spike rate 500 pA (Hz) | 46.07 ± 1.71 | 34.71 ± 2.14 | 0.0015 |
| Spike rate 400 pA (Hz) | 42.5 ± 2.15 | 33.36 ± 1.97 | 0.01 |
| Spike rate 300 pA (Hz) | 35.53 ± 2.80 | 28.75 ± 2.48 | 0.21 |
| Spike rate 200 pA (Hz) | 28.97 ± 2.46 | 22.39 ± 2.12 | 0.15 |
| Spike rate 100 pA (Hz) | 14.64 ± 2.09 | 13.92 ± 2.10 | 0.99 |
| First ISI (ms) | 17.67 ± 1.73 | 17.92 ± 2.14 | 0.99 |
| Second ISI (ms) | 19.25 ± 1.76 | 23.97 ± 2.59 | 0.75 |
| Third ISI (ms) | 21.31 ± 1.49 | 29.72 ± 2.48 | 0.96 |
| Fourth ISI (ms) | 22.83 ± 1.59 | 32.74 ± 3.55 | 0.18 |
| First spike amplitude (mV) | 75.80 ± 4.65 | 75.21 ± 4.52 | 0.99 |
| Second spike amplitude (mV) | 66.28 ± 4.37 | 59.86 ± 4.39 | 0.94 |
| First spike duration (ms) | 1.81 ± 0.12 | 1.69 ± 0.08 | 0.96 |
| Second spike duration (ms) | 2.51 ± 0.16 | 2.40 ± 0.13 | 0.98 |
aData are mean ± SEM, statistical comparisons by Student's t test and ANOVA.