Table 1.
Statistical analysis per figure
| Figure | Test | Post hoc comparison |
|---|---|---|
| 1B | Two-way ANOVA Interaction F(1,28) = 0.71 p = 0.41 DAMGO effect F(1,28) = 188 p = 6 × 10−14 SCI effect F (1,28) = 7.91 p = 0.0089 |
Sidak's multiple comparisons test *p = 0.03 at Fsk+Dm |
| 1C | Two-way ANOVA Interaction F(1,246) = 3.89 p = 0.001 DAMGO effect F(1,246) = 178.65 p = 1 × 10−86 SCI effect F(1,246) = 93.4p = 6 × 10−19 |
Sidak's multiple comparisons test At DAMGO 0.01 μM *p = 0.032; 0.03 μM ****p = 2 × 10−8; 0.1 μM ****p = 1 × 10−6; 0.3 μM **p = 0.0048; 1 μM ***p = 1 × 10−4; 3 μM **p = 0.0045 |
| 1D | Mann–Whitney test U = 34, p = 2 × 10−5 |
|
| 2D | Two-way ANOVA Interaction F(5,24) = 0.40 p = 0.84 Between clusters F(5,24) = 8.43 p = 1 × 10−4 Naive vs SCI F(1,24) = 6 × 10−5p = 0.99 |
Sidak's multiple comparisons test |
| 2E | Paired t test (two tailed), DAMGO effect Naive: CGRP (t(2) = 7.34, p = 0.081), IB-CG (t(2) = 7.23, p = 0.019), IB4 (t(2) = 6.52, p = 0.023) |
|
| 2F | Paired t test (DAMGO Effect) 2F_SCI: CGRP (t(2) = 8.17, p = 0.015) |
|
| 2F* | Unpaired t test (SCI effect) [Fsk +DAMGO] Naive vs SCI: IB4 (t(4) = 4.15, p = 0.014). |
|
| 3A | Two-way RM ANOVA Interaction F(3,60) = 3.67 p = 0.017 K+ effect F(3,60) = 165 p = 6.19 × 10−29 SCI effect F(1,20) = 5.4 p = 0.031 |
Sidak's multiple comparisons test **p = 0.0081 at 3 mM; *p = 0.039 at 10 mM |
| 3B | Two-way ANOVA Interaction F(4,46) = 1.14 p = 0.35 K+ effect F(4,46) = 53.51 p = 1 × 10−17 SCI effect F(1,46) = 10.51 p = 0.0022 |
Sidak's multiple comparisons test |
| 3C | Two-way ANOVA Interaction F(4,46) = 0.079 p = 0.99 K+ effect F(4,46) = 6.4 p = 4 × 10−4 SCI effect F(1,46) = 0.25 p = 0.62 |
Sidak's multiple comparisons test |
| 3D | Two-way ANOVA Interaction F(1,8) = 4.87 p = 0.058 IB4 effect F(1,8) = 19.55 p = 0.0022 SCI effect F(1,8) = 3 p = 0.12 |
Sidak's multiple comparisons test (Naive vs SCI) IB4+: *p = 0.047 |
| 3E | Two-way ANOVA Interaction F(2,30) = 3.7 p = 0.036 Stimuli effect F(2,30) = 120.2 p = 5 × 10−15 K+ effect F(1,30) = 14.63 p = 6 × 10−4 |
Sidak's multiple comparisons test [Control]: p = 0.041; [Fsk+DAMGO]: p = 0.0015 |
| 3F | Two-way ANOVA Interaction F(6,158) = 13.35 p = 3 × 10−12 DAMGO effect F(6,158) = 90.18 p = 2 × 10−48 K+ effect F(1,158) = 211.8 p = 6 × 10−31 |
Sidak's multiple comparisons test At DAMGO 0.03 μM ****p = 4 × 10−5; 0.1 μM ****p = 4 × 10−8; 0.3 μM ****p = 1 × 10−12; 1 μM ****p = 2 × 10−15; 3 μM ****p < 0.0001 |
| 3G | Two-way ANOVA Interaction F(6,49) = 13.35 p = 0.55 DAMGO effectF(6,49) = 90.18 p = 1 × 10−11 K+ effect F(1,49) = 211.8 p = 9 × 10−4 |
Sidak's multiple comparisons test |
| 4A | One-way ANOVA F(7,47) = 7.54, p = 4 × 10−6 |
Sidak's multiple comparisons test (vs [Control] group) [−]: ***p = 5 x 10-4, [Srci]: *p = 0.014, [PKCi] *p = 0.015, [PKAi] ****p = 7 × 10−6, [MEKi] ***p = 2 × 10−4 |
| 4A# | One-way ANOVA F(6,37) = 7.54, p = 8 × 10−4 |
Dunnett's multiple comparisons test (vs [−] group) C-Rafi ##p = 0.0082 |
| 4B | Two-way ANOVA Interaction F(15,266) = 11.2 p = 5 × 10−21 DAMGO effect F(5,266) = 191.4 p = 6 × 10−86 Treatment effect F(3,266) = 133.3 p = 1 × 10−52 |
Tukey's multiple comparisons test. [Control] vs [K+15]: p = 1 × 10−13, [Control] vs [K+15 + GW5074]: p = 2 × 10−3, [K+15] vs [K+15+GW5074]: p = 1 × 10−4 |
| 4B | Two-way ANOVA Interaction F(10,229) = 13.25p = 3 × 10−18 DAMGO effect F (5,229) = 118.64 p = 2 × 10−61 Treatment effect F(2,229) = 163.3 p = 9 × 10−45 |
Sidak's multiple comparisons test (Control + GW5074 excluded) [Control] vs [K+15]: 0.03 μM p = 3 × 10−5; 0.1 μM p = 1 × 10−12; 0.3 μM p = 4 × 10−15; 1 μM p < 0.0001; 3 μM p < 0.0001 [K+15] vs [K+15+GW5074]: 0.1 μM p = 5 × 10−5. [Control] vs [K+15+GW5074]: 0.3 μM p = 0.0015; 1 μM p = 6 × 10−15; 3 μM p = 7 × 10−8 |
| 4C | One-way ANOVA F(2,24) = 6.07, p = 0.0074 |
Tukey's multiple comparisons test Control vs K+15(–): p = 0.013; K+15(–) vs K+(GW): p = 0.034 |
| 4D | One-way ANOVA F(2,25) = 42.94, p = 8 × 10−9 |
Tukey's multiple comparisons test Control vs K+15(–): 3 × 10−8; Control vs K+(GW): 4 × 10−6 |
| 5C | Unpaired t test (two-tailed) t(9) = 3.57, p = 0.038. |
|
| 5D | One-way ANOVA F(4,15) = 14.31, p = 5 × 10−6 |
Tukey's multiple comparisons test N vs IB4: p = 1 × 10−4, CGRP vs IB4: p = 8 × 10−5, IB-CG vs IB4: p = 9 × 10−4, L,XL vs IB4: p = 0.001 |
| 5E | One-way ANOVA F(4,10) = 7.68, p = 0.0044 |
Tukey's multiple comparisons test N vs IB4: p = 0.017, CGRP vs IB4: p = 0.013, IB-CG vs IB4: p = 0.033, L,XL vs IB4: p = 0.0035 |
| 6A | Two-way ANOVA (0.01 μM point excluded) Interaction F(20,185) = 3.28 p = 1 × 10−5 DAMGO effect F(5,185) = 669.2 p = 2 × 10−116 Treatment effect F(4,185) = 15.94 p = 3 × 10−11 |
Sidak's multiple comparisons test [Control] vs [Serum]: 0.03 μM ^^^^p = 4 × 10−6; 0.1 μM ^^^p = 4 × 10−5; [Control] vs[ Raf-CTH]: 0.03 μM ***p = 5 × 10−4; 0.1 μM **p = 0.0062; 0.3 μM *p = 0.024 [Serum] vs [Ser+GW]: 0.03 μM vvvv p = 1 × 10−8; 0.1 μM vvvvp = 8 × 10−5; 0.3 μM vp = 0.043 |
| 6B | One-way ANOVA F(5,31) = 6.72, p = 2 × 10−4 |
Sidak's multiple comparisons test Control vs Serum: p = 0.016, Control vs Raf-CTH: p = 0.0028, Serum vs Ser+GW: p = 0.026 |
| 6C | One-way ANOVA F(5,30) = 103.51, p = 6 × 10−18 |
Sidak's multiple comparisons test Control vs K+15: p = 4 × 10−4, Control vs K+50: p < 0.0001, Serum vs Ser+GW: p = 0.024 |
| 7A | Two-way ANOVA Interaction F(14,158) = 1.79 p = 0.044 DAMGO effect F(7,158) = 89.08 p = 1 × 10−51 Treatment effect F(2,158) = 12.99 p = 6 × 10−6 |
Sidak's multiple comparisons test (Treatment effects) SCI vs GW5074: p = 9 × 10−5, SCI vs Retigabine: p = 1 × 10−4 |
| 7B | Two-way ANOVA Interaction F(14,158) = 1.79 p = 0.044 DAMGO effect F(7,158) = 89.08 p = 1 × 10−51 Treatment effect F(2,158) = 12.99 p = 6 × 10−6 |
Sidak's multiple comparisons test (Individual data points) SCI vs GW5074: 0.01 μM ^^p = 0.0054; 0.03 μM ^^p = 0.0034; 0.1 μM ^^p = 0.0087 SCI vs Retigabine: 0.01 μM *p = 0.022; 0.03 μM **p = 0.0025 |
| 7C | One-way ANOVA F(2,19) = 5.69, p = 0.012 |
Dunnett's multiple comparisons test Veh vs GW: p = 0.028, Veh vs Ret: p = 0.019 |
| 7D | Fisher's exact test | Bonferroni correction for two comparisons, significance levels: *p < 0.025, **p < 0.005,***p < 0.0005 At rest Veh vs GW: *p = 0.0059 Veh vs UO126: *p = 0.0059 At -45 mV Veh vs GW: ***p = 0.0002 Veh vs UO126: **p = 0.0007 |
| 7E | Brown-Forsythe and Welch ANOVA tests, respectively: F(2,30.80) = 6.18, p = 0.0055 W (2,20.56) = 5.377, p = 0.0132 |
Dunnett's multiple comparisons test Veh vs GW: p = 0.011 Veh vs UO126: p = 0.035 |
| 7F | At rest: One-way ANOVA F(2,25) = 3.616, p = 0.0418 At -45 mV: Kruskal-Wallis test p = 0.0086 |
At rest: Holm–Sidak's multiple comparison test Veh vs GW: p = 0.055 Veh vs UO126: p = 0.055 At -45 mV: Dunn's multiple comparison test Veh vs GW: p = 0.034 Veh vs UO126: p = 0.013 |