Table 1.

Characterisation of 3D liver spheroids in the presence and absence of agarose.

HepG2 cells	No Agarose		Agarose
	Day 7	Day 10	Day 7	Day 10
% Viability (95% CI)	73.5% (90.07–56.03)	60.09% (93.99–26.20)	89.98% (102.03–77.95)	79.6% (102.42–56.82)
Albumin per spheroid (ng/µl) (95% CI)	32.56 (39.17–25.94)	29.83 (33.50–26.15)	34.225 (63.99–4.46)	48.505 (94.820–2.19)
Urea per spheroid (ng/µl) (95% CI)	0.64 (0.70–0.58)	0.63 (0.68–0.58)	0.66 (0.79–0.52)	0.82* (1.09–0.54)

*Significant at P < 0.014. CI, confidence interval.