Fig. 1.

Profiling of PGC-1α–bound RNA using seCLIP. (A) PGC-1α protein contains an RNA recognition motif at its C terminus. (B) Pipeline for detecting RNAs bound to PGC-1α in hepatocytes following glucagon stimulation and using single-end cross-linking and immunoprecipitation. (C) Immunoblots depicting the upper and lower PGC-1α complexes formed following UV cross-linking and immunoprecipitation. The fragments of the IP and input that were used for RNA sequencing are marked in red. (D) Orange G staining indicates generation of suitable libraries for sequence analysis.