Fig. 4.

TRPV1 and TRPV4 are critical for fever-mediated Th2-skewed reprogramming of responding NCD4 T cells (A). Fold change in Gata3 mRNA levels in the presence or absence of TRPV inhibitors as indicated at 37 °C or 39 °C. NCD4 cells were stimulated for 6 h with plate-bound anti-CD3+anti-CD28. Fold increases in levels above those in cells activated at 37 °C without any TRPV agonists are shown. Unt, untreated with any TRPV agonist. Mean ± SE, n = 3. (B and C) Levels of secreted IL13 (B) and IFNg (C) in 24-h culture supertatants after restimulation of NCD4 cells primed at 37 °C or 39 °C in the presence or absence of TRPV1 or TRPV4 inhibitors (Inhb) for 3 d. Mean ± SE, n = 3. **P < 0.001; ***P < 0.0001.