Fig. 6.

Fever-mediated Th2-skewed programming of responding CD4 T cells is blocked by fever-mediated IL12 production from APCs (A and B) IL13 (A) and IFNg (B) levels in culture supernatants during recall response of OT-II NCD4 T cells when activated at 37 °C or 39 °C in the presence of BMDCs and Ova2 peptide. Mean ± SE; n = 3. (C) Change in IL12p40 mRNA levels of different cell types either cultured alone or purified from cocultures as indicated after activation at 37 °C or 39 °C for 6 h. mRNA levels for DCs were normalized to unstimulated BMDCs cultured at 37 °C whereas those for T cells were normalized to unstimulated NCD4 T cells. Mean ± SE; n = 3. (D and E) IL13 (D) and IFNg (E) levels in culture supernatants during recall response of OT-II NCD4 T cells when activated at 37 °C or 39 °C with plate-bound anti-CD3+anti-CD28 with or without added rIL12 during priming. Mean ± SE; n = 3. (F and G) IL13 (F) and IFNg (G) levels in culture supernatants during recall response of OT-II NCD4 T cells when activated at 37 °C or 39 °C in the presence of BMDCs and Ova2 peptide with or without anti-IL12 added during priming. Mean ± SE; n = 3. (H) IL12 levels in culture supernatants during recall response of OT-II NCD4 T cells when activated at 37 °C or 39 °C in the presence of either BMDCs or LPS-activated B cells as APCs and Ova2 peptide during priming. Mean ± SE; n = 3. (I and J) IL13 (I) and IFNg (J) levels in culture supernatants during recall response of OT-II NCD4 T cells when activated at 37 °C or 39 °C in the presence of either BMDCs or LPS-activated B cells as APCs and Ova2 peptide during priming cultures. Mean ± SE; n = 3. **P < 0.001; ***P < 0.0001.