Fig. 2.

Autoantibody redemption requires a path for escape of self-reactivity. (A) Mutational trajectories of Hy10-expressing SW_HEL B cells upon immunization with foreign or self-antigen (day 15). CD45.1⁺ GC B cells were subjected to single-cell sequencing. Dashed lines show the affinities of the WT founder antibody for self and foreign proteins. Circles show the affinities of recurring mutant antibodies for self and foreign proteins. Areas of the circles denote the percentages of CD45.1⁺ GC B cells with the indicated mutations. Arrows indicate predominant mutational trajectories in the presence (Left) or absence (Right) of membrane-bound HEL^3X self-lysozyme. (B) Percentage of CD45.1⁺ GC cells with substitutions at antibody variable heavy-chain positions. Data are pooled from two experiments per time point with three to four mice per group.