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. 2020 Sep 11;11(9):741. doi: 10.1038/s41419-020-02967-z

Fig. 5. Ectopic IGF2BP1 overexpression is ineffective on the functions of LIN28B-AS1 KO HepG2 cells.

Fig. 5

The stable HepG2 cells with CRISPR/Cas9-LIN28B-AS1-KO construct (“KO-LIN28B-AS1”) were further infected with recombinant adenovirus encoding the human IGF2BP1 expression construct (“Ad-IGF2BP1”) or empty vector (“Vec”), control cells were transduced with the CRISPR/Cas9 control construct (“CRISPR-C”); Expression of listed proteins a and LIN28B-AS1 b was shown; Cells were further cultured for 48 h, cell proliferation and apoptosis were tested by EdU incorporation c and TUNEL staining d assays, respectively. Expression of IGF2BP1 and Tubulin in stable HepG2 cells with the CRISPR/Cas9-IGF2BP1-KO construct (IGF2BP1-KO cells) or control cells (“Ctrl”) was shown e; IGF2BP1-KO cells were further transfected with LIN28B-AS1 siRNA (“si-LIN28B-AS1-S1”, 0.5 μM) or LV-LIN28B-AS1 for 48 h, cell proliferation f and apoptosis g were tested similarly. Listed proteins were quantified and normalized a. Data were presented as mean ± standard deviation (SD, n = 5). The experiments were repeated three times, and similar results were obtained. Bar = 100 μm.