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. 2020 Sep 11;11(9):742. doi: 10.1038/s41419-020-02925-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic model of the co-culture system of macrophages and NPC cells; EB⁺CNE1 cells stably expressing shNC or shATR were co-cultured with M0 macrophages for 2 days, and EB⁻ and EB⁺ were used as negative and positive controls, respectively. b qPCR determined mRNA levels of M1 and M2 macrophage markers; c Detection of the expression of CD68⁺/CD206⁺ by FCM. d WB detection of protein expression of CD68, CD86, and CD206 at different time points after co-culture. Data are represented as the mean ± SD of three independent experiments. *p < 0.05.