KEY RESOURCES TABLE
Resource Type | Specific Reagent or Resource | Source or Reference | Identifiers | Additional Information |
---|---|---|---|---|
Add additional rows as needed for each resource type | Include species and sex when applicable. | Include name of manufacturer, company, repository, individual, or research lab. Include PMID or DOI for references; use “this paper” if new. | Include catalog numbers, stock numbers, database IDs or accession numbers, and/or RRIDs. RRIDs are highly encouraged; search for RRIDs at https://scicrunch.org/resources. | Include any additional information or notes if necessary. |
Antibody | Cat # MAB305 | |||
Bacterial or Viral Strain | University of Pennsylvania, Viral Vector Core | N/A | ||
Biological Sample | ||||
Cell Line | ||||
Chemical Compound or Drug | ||||
Commercial Assay Or Kit | ||||
Deposited Data; Public Database | ||||
Genetic Reagent | ||||
Organism/Strain | rat: Long Evans-Tg(ChAT-Cre)5.1Deis, male and female | Rat Resource and Research Center | ||
Organism/Strain | rat: Sprague Dawley, male and female | Charles River | ||
Organism/Strain | rat: Long-Evans, male and female | Charles River | RRID:RGD_2308852 | |
Peptide, Recombinant Protein | Choline oxidase from Alcaligenes Sp. (E.C.1.1.3.17) | Millipore Sigma | Cat # C5896 | |
Recombinant DNA | ||||
Sequence-Based Reagent | ||||
Software; Algorithm | Neurolucida | MBF Bioscience Inc. | RRID:SCR_001775 | |
Software; Algorithm | FAST-16 data acquisition | Quanteon | N/A | |
Transfected Construct | ||||
Commercial Assay Or Kit | iScript cDNA Synthesis Kit | Bio-Rad | 1708891BUN | |
Commercial Assay Or Kit | RNeasy Mini Kit | Qiagen | 74106 | |
Commercial Assay Or Kit | Universal RNA-Seq Library Preparation Kit | TECAN | Part No. 7102 | |
Sequence-Based Reagent | Primers for RT-qPCR, see Supplement methods | This paper | N/A | |
Software; Algorithm | STAR | PMID: 23104886 | STAR/2.5.2a | |
Software; Algorithm | HTSeq Python package | PMID: 25260700 | HTseq-count | |
Software; Algorithm | DESeq | PMID: 20979621 | ||
Deposited data | GSE147806 | NCBI GEO DataSets | https://www.ncbi.nlm.nih.gov/gds | |
Software; Algorithm | samtools | PMID: 19505943 | samtools-1.1 | |
Software; Algorithm | bedtools | PMID: 25199790 | ||
Other |