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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Biol Psychiatry. 2020 Apr 28;88(7):566–575. doi: 10.1016/j.biopsych.2020.04.013

KEY RESOURCES TABLE

Resource Type Specific Reagent or Resource Source or Reference Identifiers Additional Information
Add additional rows as needed for each resource type Include species and sex when applicable. Include name of manufacturer, company, repository, individual, or research lab. Include PMID or DOI for references; use “this paper” if new. Include catalog numbers, stock numbers, database IDs or accession numbers, and/or RRIDs. RRIDs are highly encouraged; search for RRIDs at https://scicrunch.org/resources. Include any additional information or notes if necessary.
Antibody mouse anti-ChAT monoclonal antibody Millipore Cat # MAB305
Bacterial or Viral Strain AAV9.CAG.Flex.eGFP.WPRE.bGH University of Pennsylvania, Viral Vector Core N/A
Biological Sample
Cell Line
Chemical Compound or Drug 2,4,5-Trimethylthiazole Sigma Aldrich Cat # W332518–25G-K
Commercial Assay Or Kit
Deposited Data; Public Database
Genetic Reagent
Organism/Strain rat: Long Evans-Tg(ChAT-Cre)5.1Deis, male and female Rat Resource and Research Center RRID:RGD_10401204
Organism/Strain rat: Sprague Dawley, male and female Charles River RRID:RGD_10395233
Organism/Strain rat: Long-Evans, male and female Charles River RRID:RGD_2308852
Peptide, Recombinant Protein Choline oxidase from Alcaligenes Sp. (E.C.1.1.3.17) Millipore Sigma Cat # C5896
Recombinant DNA
Sequence-Based Reagent
Software; Algorithm Neurolucida MBF Bioscience Inc. RRID:SCR_001775
Software; Algorithm FAST-16 data acquisition Quanteon N/A
Transfected Construct
Commercial Assay Or Kit iScript cDNA Synthesis Kit Bio-Rad 1708891BUN
Commercial Assay Or Kit RNeasy Mini Kit Qiagen 74106
Commercial Assay Or Kit Universal RNA-Seq Library Preparation Kit TECAN Part No. 7102
Sequence-Based Reagent Primers for RT-qPCR, see Supplement methods This paper N/A
Software; Algorithm STAR PMID: 23104886 STAR/2.5.2a
Software; Algorithm HTSeq Python package PMID: 25260700 HTseq-count
Software; Algorithm DESeq PMID: 20979621
Deposited data GSE147806 NCBI GEO DataSets https://www.ncbi.nlm.nih.gov/gds
Software; Algorithm samtools PMID: 19505943  samtools-1.1
Software; Algorithm bedtools PMID: 25199790
Other