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. 2020 Sep 10;8:e9917. doi: 10.7717/peerj.9917

Figure 2. Biofilm formation of BV-associated bacteria in the nine different culture media over a 72 h period.

Figure 2

Biofilm biomass was quantified using the crystal violet staining assay. (A) The total biofilm biomass formed by Gardnerella sp. in the tested culture media. (B) The total biofilm biomass formed by A. vaginae. (C) The total biofilm biomass formed by L. iners. (D) The total biofilm biomass formed by M. curtisii. (E) The total biofilm biomass formed by P. anaerobius. (F) The total biofilm biomass formed by P. bivia. Results are expressed as the average ± standard deviation of at least three independent experiments performed with eight technical replicates. Statistical analysis was performed using one-way ANOVA and Dunnett’s multiple comparisons test. Significant differences between biofilm biomass formed in NYC medium (our culture medium of choice) and other culture media are represented with *p < 0.05 and **p < 0.01. Other statistical analysis is shown in Table S2.