Table 1. Cultivable bacterial species used for planktonic and biofilm growth assays.
Species | Strain | Origin | Association with BV1 |
---|---|---|---|
Gardnerella sp. | UM2412 | Isolated from women diagnosed with BV | Often described |
Atopobium vaginae | ATCC BAA-55T | Isolated from vaginal microbiota of a healthy woman (Jovita et al., 1999) | Often described |
Lactobacillus iners | CCUG 28746T | Isolated from human urine (Falsen et al., 1999) | Commonly described |
Mobiluncus curtisii | ATCC 35241T | Isolated from women with BV (Spiegel & Roberts, 1984) | Commonly described |
Peptostreptococcus anaerobius | ATCC 27337T | Isolated from female genital tract (Ng et al., 1994) | Commonly described |
Prevotella bivia | ATCC 29303T | Isolated from endometrium (Holdeman & Johnson, 1977) | Commonly described |
Notes:
To determine how often the selected cultivable species have been reported in BV, a query in PubMed was performed by using a specific combination of keywords as “bacterial vaginosis” and “name of each species” (e.g., “Gardnerella”and “bacterial vaginosis”). We designated as “often described” those species referred in more than 50 articles in the last 25 years, while “commonly described” had at least 10 articles in the same period. Of note, the selected bacterial species used herein have been pointed out in several studies (Diop et al., 2019; Onderdonk, Delaney & Fichorova, 2016; Ravel et al., 2011) as potential microbial pathogens involved in BV development.
The partial 16S ribosomal RNA gene sequences of Gardnerella sp. is downloadable from NCBI. UM: University of Minho, Portugal. In addition, the strain was phenotypically and genotypically characterized by Castro et al. (2020, 2015), Castro, Jefferson & Cerca (2018). It is of note that Gardnerella sp. UM241 did not match with any of the Gardnerella species described by Vaneechoutte and colleagues (Vaneechoutte et al., 2019) (i.e., G. vaginalis, G. piotii, G. leopoldii and G. swidsinskii), belonging to a yet unidentified Gardnerella species (Castro et al., 2020).