Table 2. Culture media used for the growth of BV-associated anaerobes.
| Culture medium | Composition | Supplementation | Abbreviation |
|---|---|---|---|
| Brain heart infusion broth (Liofilchem, Italy) | As described by the manufacturer | 2% (w/w) Gelatine (Liofilchem, Italy); 0.1% (w/w) Starch (Panreac, Spain); 0.5% (w/w) Yeast extract (Liofilchem, Italy) |
sBHI/sBHI.Aa1 |
| Brucella broth (Liofilchem, Italy) |
As described by the manufacturer | 0.0005% (w/v) Hemin (Sigma, China); 0.0001% (w/v) Vitamin K1 (Sigma, China) |
BHV/BHV.Aa1 |
| New York City III broth | 1.5% (w/v) Bacto proteose peptone no. 3 (BD, France); 0.5% (w/v) Glucose (Fisher Scientific, UK); 0.24% (w/v) HEPES (VWR, USA); 0.5% (w/v) NaCl (VWR, USA); 0.38% (w/v) Yeast extract (Liofilchem, Italy) |
10% (v/v) Inactivated horse serum (Biowest, France) |
NYC/NYC.Aa1 |
| Schaedler broth (Liofilchem, Italy) | As described by the manufacturer | – | SB/SB.Aa1 |
| Chemically defined medium simulating genital tract secretions (Stingley et al., 2014) | Part I: 0.35% NaCl; 0.15% KCl; 0.174% K2HPO4; 0.136% KH2PO4; 1.08% glucose; 0.05% cysteine HCl. Part II: 0.1% glycogen; 0.03% mucin; 0.02% tween 20; 0.05% urea; 0.0005% hemin; 0.0001% vitamin K1; 0.2% bovine serum albumin; 0.03% MgSO4; 0.004% NaHCO3; 0.1% sodium acetate; 0.005% MnCl2. Part III: 0.0005% biotin; 5.0% myo-inositol; 0.05% niacinamide; 0.05% pyridoxine HCI; 0.05% thiamin HCI; 0.05% D-calcium pantothenate; 0.05% folic acid; 0.001% p-aminobenzoic acid; 0.05% choline chloride; 0.01% riboflavin; 0.1% L-ascorbic acid; 0.0005% vitamin A (retinol); 0.0005% vitamin D (cholecalciferol); 0.001% vitamin B12. Part IV (amino acids): 0.032% alanine; 0.008% arginine; 0.076% aspartic acid; 0.036% glutamic acid; 0.04% glutamine; 0.02% glycine; 0.016% histidine; 0.012% isoleucine; 0.02% leucine; 0.02% lysine; 0.004% methionine; 0.004% phenylalanine; 0.028% proline; 0.012% serine; 0.012% threonine; 0.004% tryptophan; 0.02% tyrosine; 0.068% valine. Part V (UPI): 0.05% uracil; 0.01% sodium pyruvate; 0.02% inosine. |
– | mGTS2 |
Notes:
1
The supplementation of the culture media with 0.1% (w/v) L-ascorbic acid was designated with the abbreviation “Aa”, added at the end of each medium’s name as follows, for example, sBHI supplemented with L-ascorbic acid became sBHI.Aa. The other three culture media were abbreviated by following the same rule.
2
The mGTS medium has already 0.1% (w/v) L-ascorbic acid in its composition.