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. 2020 Sep 3;5(17):e136215. doi: 10.1172/jci.insight.136215

Figure 6. Steatosis promotes metastatic tumor growth.

Figure 6

(A) BrdU incorporation and (B) AKT and cyclin D1 immunoblot of Bo1 cells treated with lipids (100 μg/mL) from control or FF liver. n = 5. (C) Seahorse oxygen consumption rate (OCR) of Bo1 cells cocultured with control or fatty liver. n = 9. (D) Carnitine palmitoyltransferase 1 (CPT1) mRNA expression in Bo1 cells cocultured with control or FF liver. n = 4–5. (E) BLI analysis of FF liver 12 days after intracardiac injection of CRISPR control or CRISPR-CPT1–knockout Bo1 cells. n = 4–7. (F) Bo1 cells were cultured with control or FF liver explants in Transwell system and migrating cell number was analyzed. n = 4. (G and H) Two-month-old FF and control mice were injected intracardiacally with Bo1 cells. Tumor burden was analyzed by BLI 24 hours later: (G) ex vivo image of liver and (H) quantification of tumor burden in liver. n = 6. Data are presented as mean ± SD. *P < 0.05, ***P < 0.001 as determined by unpaired 2-tailed t test (A, C, D, F, and H) or 1-way (E) ANOVA test with analysis of variance with Holm-Šidák multiple-comparisons test.