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. 2020 Aug 31;8:712. doi: 10.3389/fcell.2020.00712

FIGURE 5.

FIGURE 5

Citicoline attenuates oxidative stress in cochlear HCs after neomycin exposure. (A) Immunofluorescence staining with Mito-SOX Red and anti-Myo7A antibodies in the middle turn of the cochlea after different treatments. (B) Quantification of the numbers of Mito-SOX Red and Myo7A double-positive cells in (A). (C) The mRNA levels of genes related to redox reactions were analyzed by qRT-PCR after neomycin and citicoline treatment and normalized to GAPDH and presented as the fold change compared to control levels. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars = 20 μm.