Figure 2.

Effects of high glucose on Ephrin B2 mRNA and protein expression in HAECs. (A) HAECs were cultured with increased glucose (5.5, 15, 30 mM) for 48 hours. Mannitol (MN) was used as a control hyperosmolarity. mRNA levels of Ephrin B2 were detected by qRT-PCR. (B) Representative blots for Ephrin B2. (C) Graphic presentation. Data were exhibited as means ± SD. *, P<0.05 vs. NG or MN. **, P<0.01 vs. NG or MN. Experiments were repeated five times. HAECs, human aortic endothelial cells; NG, normal glucose.