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. 2020 Aug;10(4):738–751. doi: 10.21037/cdt-20-407

Figure 4.

Figure 4

Inhibition of miR-181b drives KLF4 SUMOylation. (A) Western blots show that overexpression of miR-181b promotes the protein expression of KLF4. (B) Western blots show that knockdown of miR-181b suppresses the protein expression of KLF4. (C) Quantitative PCR shows that overexpression of miR-181b does not the mRNA expression of KLF4. (D) Proteasome inhibitor MG132 reverses the protein expression of KLF4 reduced by miR-181b inhibitors (anti181b). (E) Endogenous SUMO1 conjugates of KLF4 are determined by co-immunoprecipitation in the lysates of macrophages transfected with anti181b. Human macrophages from circulating monocytes were maintained in 20% FBS/RPMI with recombinant M-CSF (100 ng/mL). Each experiment was performed in triplicate. Data are presented as mean ± SD.