MethyLight |
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MiR-181b
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Forward: 5'-GGA ATG TAA GAG TAT TTA TTA GCG G-3' |
Reverse: 5'-ACC CCA AAA TAC AAT CAA CGA T-3' |
ACTB
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Forward: 5'-TGG TGA TGG AGG AGG TTT AGT AAG T-3’ |
6FAM-ACCACCACCCAACACACAATAACAAACACA-BHQ1 |
Reverse: 5'-AAC CAA TAA AAC CTA CTC CTC CCT TAA-3' |
Pyrosequencing |
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MiR-181b
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Forward: 5'-GAT TTG AAA TTT AGA GAG GAA TGT AAG AG-3' |
5’-ATTTGAAATTTAGAGAGGAATGTAAGAGTA-3’ |
Reverse: 5'-ATA AAA ACC CCA AAA TAC AAT CAA C-3' |
MiR-181b-LUC-KpnI
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Forward: 5'-GGT ACC GTA AGA GCA TCC ATC |
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Reverse: 5'-GGT ACC GTA CAG TCA ACG GTC AGT G |
Quantitative PCR |
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TNF-α
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Forward: 5'-CCT CTC TCT AAT CAG CCC TCT G-3' |
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Reverse: 5'-GAG GAC CTG GGA GTA GAT GAG-3' |
IL-6
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Forward: 5'-ACT CAC CTC TTC AGA ACG AAT TG-3' |
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Reverse: 5'-CCA TCT TTG GAA GGT TCA GGT TG-3' |
Arg-1
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Forward: 5'-GTG GAA ACT TGC ATG GAC AAC-3' |
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Reverse: 5'-AAT CCT GGC ACA TCG GGA ATC-3' |
CD206
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Forward: 5'-TCC GGG TGC TGT TCT CCT A-3' |
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Reverse: 5'-CCA GTC TGT TTT TGA TGG CAC T-3' |
GADPH
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Forward: 5'-GGA GCG AGA TCC CTC CAA AAT-3' |
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Reverse: 5'-GGC TGT TGT CAT ACT TCT CAT GG-3' |