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. 2020 Aug;10(4):738–751. doi: 10.21037/cdt-20-407

Table S1. Summary of quantitative RT-PCR and MethyLight primer and probe sequences.

Gene (Ref.) Primer (5'→3') Probe
MethyLight
   MiR-181b Forward: 5'-GGA ATG TAA GAG TAT TTA TTA GCG G-3'
Reverse: 5'-ACC CCA AAA TAC AAT CAA CGA T-3'
   ACTB Forward: 5'-TGG TGA TGG AGG AGG TTT AGT AAG T-3’ 6FAM-ACCACCACCCAACACACAATAACAAACACA-BHQ1
Reverse: 5'-AAC CAA TAA AAC CTA CTC CTC CCT TAA-3'
Pyrosequencing
   MiR-181b Forward: 5'-GAT TTG AAA TTT AGA GAG GAA TGT AAG AG-3' 5’-ATTTGAAATTTAGAGAGGAATGTAAGAGTA-3’
Reverse: 5'-ATA AAA ACC CCA AAA TAC AAT CAA C-3'
   MiR-181b-LUC-KpnI Forward: 5'-GGT ACC GTA AGA GCA TCC ATC
Reverse: 5'-GGT ACC GTA CAG TCA ACG GTC AGT G
Quantitative PCR
   TNF-α Forward: 5'-CCT CTC TCT AAT CAG CCC TCT G-3'
Reverse: 5'-GAG GAC CTG GGA GTA GAT GAG-3'
   IL-6 Forward: 5'-ACT CAC CTC TTC AGA ACG AAT TG-3'
Reverse: 5'-CCA TCT TTG GAA GGT TCA GGT TG-3'
   Arg-1 Forward: 5'-GTG GAA ACT TGC ATG GAC AAC-3'
Reverse: 5'-AAT CCT GGC ACA TCG GGA ATC-3'
   CD206 Forward: 5'-TCC GGG TGC TGT TCT CCT A-3'
Reverse: 5'-CCA GTC TGT TTT TGA TGG CAC T-3'
   GADPH Forward: 5'-GGA GCG AGA TCC CTC CAA AAT-3'
Reverse: 5'-GGC TGT TGT CAT ACT TCT CAT GG-3'