FIGURE 11.

Lack of Dp427 determines a reduction in the number of cleaved caspase-3-immunopositive apoptotic cells during retinogenesis of P10 mdx mice compared to wild type. (A) Representative images of horizontal (N–T) retina cryosections of P0, P5, and P10 wild type (wt) and mdx mice immunolabeled for cleaved caspase-3 (CC-3). Nuclei are stained in blue (Hoechst). (B,C) Comparative quantitative analysis of the number of CC-3⁺ cells/section between wt and mdx age-matched mice (B) and across post-natal days within the same genotype (C). Counts are made along the entire retinal perimeter. Data are analyzed by either two-way ANOVA test Sidak post hoc (B) or one-way ANOVA test Tukey post hoc (C) and represented as the mean ± SEM. ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001 (mdx vs. wt; P5, P10 vs. P0); ^#p ≤ 0.05 (P10 vs. P5). n = 3–5 independent experiments. Scale bar: 50 μm; insets: 10 μm. OS, outer segment of photoreceptors; IS, inner segment of photoreceptors; NBL, neuroblastic cell layer; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.