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. 2020 Aug 31;14:760. doi: 10.3389/fnins.2020.00760

FIGURE 3.

FIGURE 3

Lack of Dp427 does not alter the number of calbindin-immunopositive horizontal cells. (A) Representative images of horizontal (N–T) retina cryosections of P0, P5, P10, and 6–7-week-old wild type (wt) and mdx mice immunolabeled for calbindin (CALB). Nuclei are stained in blue (Hoechst). (B,C) Quantitative analysis of the number of CALB+ cells/mm of retina between wt and mdx age-matched mice (B) and across post-natal days within the same genotype (C). Cell counts are made in the posterior retina (black boxes in the eye drawing). Data are analyzed by either two-way ANOVA test Sidak post hoc (B) or one-way ANOVA test Tukey post hoc (C) and represented as the mean ± SEM. ∗p ≤ 0.05, ∗∗p ≤ 0.01 (vs. P0); ##p ≤ 0.01 (vs. P5). n = 3–5 independent experiments. Scale bar: 50 μm; insets: 10 μm. OS, outer segment of photoreceptors; IS, inner segment of photoreceptors; NBL, neuroblastic cell layer; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.