FIGURE 5.

Lack of Dp427 does not alter the number of glutamine synthase-immunopositive Müller cells. (A) Representative images of horizontal (N–T) retina cryosections of P10 and 6–7-week-old wild type (wt) and mdx mice immunolabeled for glutamine synthase (GS). Nuclei are stained in blue (Hoechst). (B,C) Comparative quantitative analysis of the number of GS⁺ cells/mm of retina between wt and mdx age-matched mice (B) and across post-natal days within the same genotype (C). Cell counts are made in the posterior retina (black boxes in the eye drawing). Data are analyzed by two-way ANOVA test Sidak post hoc and represented as the mean ± SEM. ^§§§p ≤ 0.001 (vs. P10). n = 3 independent experiments. Scale bar: 50 μm; insets: 10 μm. OS, outer segment of photoreceptors; IS, inner segment of photoreceptors; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.