FIGURE 8.

Lack of Dp427 determines transitory alterations in the number of GABA-immunopositive amacrine cells in P5 and P10 mdx mice compared to wild type. (A) Representative images of horizontal (N–T) retina cryosections of P5, P10, and 6–7-week-old wild type (wt) and mdx mice immunolabeled for GABA. Nuclei are stained in blue (Hoechst). (B,C) Comparative quantitative analysis of the number of GABA⁺ cells/mm of retina between wt and mdx age-matched mice (B) and across post-natal days within the same genotype (C). The counts of immunopositive amacrine cells residing in the INL and of those located in the GCL have been kept separate. Counts are made in the posterior region of the retina (black boxes in the eye drawing). Data are analyzed by either two-way ANOVA test Sidak post hoc (B) or one-way ANOVA test Tukey post hoc (C) and represented as the mean ± SEM. ^∗p ≤ 0.05, ^∗∗∗p ≤ 0.001 (mdx vs. wt); ^##p ≤ 0.01, ^###p ≤ 0.001 (vs. P5); ^§p ≤ 0.05, ^§§§p ≤ 0.001 (vs. P10). n = 3–5 independent experiments. Scale bar: 50 μm; insets: 10 μm. OS, outer segment of photoreceptors; IS, inner segment of photoreceptors; NBL, neuroblastic cell layer; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.