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. 2020 Aug 31;14:760. doi: 10.3389/fnins.2020.00760

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Copyright © 2020 Persiconi, Cosmi, Guadagno, Lupo and De Stefano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The pattern of distribution of EdU⁺ proliferative cells along the retinal perimeter does not differ between wild type and mdx mice. On the left side of the figure, representative images of EdU⁺ cell (green) distribution along the entire perimeter of horizontal (N–T) retina cryosections of P0, P5, and P10 wild type (wt) and mdx mice. Nuclei are stained in blue (Hoechst); c: cornea; l: lens. On the right side, comparative quantitative analysis of the number of EdU⁺ proliferating cells/region of interest (ROI), distinct by regions: anterior, middle and posterior retina. Data are analyzed by one-way ANOVA test Tukey post hoc and represented as the mean ± SEM. ^∗∗∗p ≤ 0.001 (vs. anterior region); ^#p ≤ 0.05 (vs. middle region). n = 3–6 independent experiments. Scale bar: 200 μm.