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. 2020 Aug 31;11:1993. doi: 10.3389/fmicb.2020.01993

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Copyright © 2020 Crestani, Forde and Zadoks.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Map of the insertion sites of prophages and putative phage-inducible chromosomal islands (PICI) in Group B Streptococcus (GBS). Twelve phage insertion sites (red arrows) and sixteen integrase types were identified; phage insertion sites indicated with “GBS” and a progressive number based on order of detection are shown (integrase type sub-number indicates the different subtypes of integrases found at the same insertion site based on <90% similarity in the amino acid sequence). GBS5 integration site corresponds to the rpsI gene, a site of integration in common with ICE (Brochet et al., 2008; Ambroset et al., 2016) and PICI-like elements (this work). The putative PICI insertion site is displayed in blue and is the same for both PICI integrases (1 and 2). Arrows show the direction of packaging.