Table 1.
Insertion site | Phage Integrase type | Phage integrase length (AA) | Gene | Putative attachment site |
---|---|---|---|---|
GBS1 | GBSInt1 | 369 | comX (sigma-70 familyRNA polymerase sigma factor)- 3′ end |
attL TTTTTTGTTATAATATAAGA attR TTTTTTGTTATAATATAATA |
GBS2 | GBSInt2.1 | 360 | tRNA methyltransferase—3′ end | ATCCCCTCCTCTCCTTTAAT |
GBSInt2.2 | 363 | |||
GBS3 | GBSInt3 | 368 | rplS—3′ end |
attL GATTCCGGCAGGGGACAT attR GATTCCAGCAGGGGACAT |
GBS4 | GBSInt4 | 389 | HU, histone-like DNA-binding protein | CTCTTAAAGACGCTGTTAAATA ATTCGTCTAGAAAAACCTTGTC ATATCAATGTTTATTGATAGCGAC AAGGTTC |
GBS5 | GBSInt5 | 331 | rpsI—3′ end (within ICE) | – |
GBS6 | GBSInt6.1 | 366 | CatB-related O-acetyltransferase—5' end | TGGAGCCGGTGGGAGT |
GBSInt6.2 | 366 | |||
GBS7 | GBSInt1 | 369 | hylB—5′ end |
attL TTTTTTGTTATAATATAAGA attR TTTTTTGTTATAATATGAGA |
GBS8 | GBSInt8 | 382 | YbaB/EbfC family nucleoid-associated protein | TTTTGCATATTCATCATA |
GBS9 | GBSInt9.1 | 360 | nhaK (sodium/proton antiporter)—3′ end | AAGGCGGTAGACGGATTTGAA |
GBSInt9.2 | 359 | |||
GBS10 | GBSInt10 | 476 | DNA-binding protein WhiA—3′ end | - |
GBS11 | GBSInt11.1 GBSInt11.2 GBSInt11.3 |
489 486 367 |
gspF or gspF+competence proteins/type II secretion system proteins—3′ end | CTTTTAGAATGTTTGGTA– |
GBS12 | GBSInt12 | 386 | 5-formyltetrahydrofolate cyclo-ligase—5′ end | - |
With the exception of GBSInt1, integrases are site specific. Putative attachment sites (att) are shown when known. For att sites that differed slightly at the two ends of the lysogenic prophages, left and right (attL and attR) sequences are specified and differences are highlighted in bold.