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. 2020 Sep 9;51:112. doi: 10.1186/s13567-020-00836-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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DNA electrophoresis analysis of the PCV2-Cap (d41) gene PCR product and confirmation of the pBacSC-Cap (d41) plasmid. A Lane M represents DNA marker (Bio-100 bp DNA ladder); lanes 1-3 represent PCR amplified PCV2-Cap (d41) gene fragments, which have restriction enzyme cleavage positions (Lane 1: XhoI/PstI, lane 2: NotI/SalI and lane 3: XhoI/BsiWI). The expected fragment size is 579 bp in length. B The pBacSC-Cap (d41) vector was cleaved by restriction enzymes XhoI and PstI to confirm the successful gene recombination, which was in line with the expected fragment size of about 579 bp in length.